In vivo characterization of streptococcus pneumoniae genes involved in the pathogenesis of meningitis by differential fluorescence induction

To further understand the pathogenesis of pneumococcal meningitis, and provide some target candidates for the development of drugs. This study was performed at the Department of Laboratory Medicine, Key Laboratory of Diagnostic Medicine [Ministry of Education], Chongqing Medical University, Chongqing, China from March 2006 to December 2007. A promoter-trap library of Streptococcus pneumoniae TIGR4, reported by green fluorescent protein was constructed, and used to infect BALB/c mice [n=15] intranasally, to set up a meningitis model. The control group [n=5] were inoculated with sterile phosphate buffered saline. The bacteria containing the promoter fusions induced only in meningitis brain tissue, not in vitro were screened by differential fluorescence induction. The obtained bacteria were prepared to re-infect the mice and re-screened, as above. The sorted bacteria were spread on trypticase soy agar with 5% sheep blood agar plates containing chloramphenicol [2.5 micro g/ml], and were used for DNA cloning, sequencing, and bioinformatics analysis. A total of 52 genes were obtained. Bioinformatics analysis revealed that these in vivo induced genes were involved in functions such as, adherence, energy metabolism, nutrient substance transport, transcription regulation, DNA metabolism, as well as, cell wall synthesis. In addition, there were some genes encoding for some hypothetical proteins with unknown, or putative functions. Pneumococcal genes involved in meningitis identified in this study are potential targets to understand the pathogenesis of pneumococcal meningitis

Gastrointestinal B-cell lymphoma: a morphologic and immunohistochemical study of 194 cases / 中华病理学杂志

<p><b>OBJECTIVE</b>To study the morphologic and immunohistochemical features of gastrointestinal B-cell lymphomas.</p><p><b>METHODS</b>One hundred and ninety-four cases of gastrointestinal B-cell lymphoma were retrieved from the archival file. The clinical features and pathologic findings were reviewed. Immunohistochemical study for B-cell markers, T-cell markers, bcl-6, CD10, bcl-10, cyclin D1, TdT, MUM1 and Ki-67 was carried out.</p><p><b>RESULTS</b>The male-to-female ratio was 1.4:1. The age of patients ranged from 8 to 85 years. Amongst the 194 cases studied, 128 (66.0%) were diagnosed as diffuse large B-cell lymphoma, including 16 cases of large cell lymphoma associated with mucosa-associated lymphoid tissue (MALT) lymphoma component. There were also 40 cases (20.6%) of MALT lymphoma, 8 cases (4.1%) of follicular lymphoma, 5 cases of (2.6%) of lymphoplasmacytic lymphoma, 3 cases (1.6%) of mantle cell lymphoma, 1 case of (0.5%) of B-lymphoblastic lymphoma and 9 cases (4.6%) of indefinite type (including 5 biopsy cases). The site of involvement included stomach (100 cases, 51.5%), small intestine (43 cases, 22.2%), ileocecal junction (26 cases, 13.4%), appendix (1 case, 0.5%), colon (21 cases, 10.8%) and rectum (3 cases, 1.6%). Amongst the 163 cases which had undergone surgical resection, 20 cases (12.3%) cases had invasion down to the mucosa, 20 cases (12.3%) down to the superficial muscular layer, 19 cases (11.6%) down to the deep muscular layer and 104 cases (63.8%) with full-thickness involvement. Histologic examination showed lymphoepithelial lesions in 52 cases, residual lymphoid follicles in 29 cases, coagulative necrosis in 66 cases and nodular growth pattern in 30 cases. The lymphoma cells in all cases were immunoreactive for B-cell marker CD20. There was also various degrees of positivity for bcl-6, CD10, bcl-10, cyclin D1, TdT, MUM1 and Ki-67.</p><p><b>CONCLUSIONS</b>Gastrointestinal B-cell lymphomas can be subdivided into two main groups: large B-cell lymphomas and small B-cell lymphomas. The latter group often poses diagnostic pitfalls. Accurate pathologic typing requires correlation with histologic and immunohistochemical findings.</p>

Study on yield and quality of Scutellaria baicalensis from different habitats / 中国中药杂志

<p><b>OBJECTIVE</b>To compare the economical characters, yield characters and major effective ingredient content of Scutellaria baicalensis from different habitats.</p><p><b>METHOD</b>The characters were studied by field randomized block test combining analysis of variance, correlation analysis and quality analysis.</p><p><b>RESULT</b>There are significant differences in yield and quality of the crude drugs from different habitats.</p><p><b>CONCLUSION</b>The crude drug from Pingyi (Shandong province) is better than those from other habitats.</p>

Development of a pathological information network and image analysis system / 中国医疗器械杂志

This paper introduces a pathological information network and image analysis system designed by ourselves. The system offers an efficient means for modern medical diagnosis and treatment, teaching, research and management in the department of pathology.

Occurrence and control of pests about Cistanche deserticola and its hosts / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the kinds and occurrence of pests of Cistanche deserticola and its hosts so as to find ways of pest control.</p><p><b>METHOD</b>Pests of C. deserticola and its hosts were investigated in Xinjiang, Inner Mongolia and Ningxia where C. deserticola grows, the occurrence of Anomala titanis Reitter was investigated in the field, and phoxim was used for field control.</p><p><b>RESULT</b>There are 17 kinds of pests which harm C. deserticola and its hosts. Phoxim 40% EC 1000 times, 2000 times and Phoxim 3% GR [8 kg x (667 m2)(-1)] had 100% controlling effect, and 3% GR [4 kg x (667 m)(-2-1)] had 88.23% controlling effect.</p><p><b>CONCLUSION</b>Many kinds of pests can harm the C. deserticola and its hosts. Phoxim is good for killing A. titanis Reitter.</p>

Study on the pollination characteristic of Cistanche deserticola / 中国中药杂志

<p><b>OBJECTIVE</b>To study the Pollination Characteristic of C. deserticola.</p><p><b>METHOD</b>Four pollination methods were used, cross pollination coming different plant, cross pollination in one plant, wind pollination and no-pollination.</p><p><b>RESULT</b>Cross pollinations coming different plant are fructiferous. The average fructication rate of cross pollination coming different plant is 94.1%; the average fructication rate of cross pollination in one plant is 52.2%; The seed qulitity of cross pollination coming different plant is better than the cross pollination in one plant.</p><p><b>CONCLUSION</b>Cross pollinations coming different plant have a high quantity and quality of seeds of C. deserticola.</p>